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Jennifer Gori
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E-mail: gori0011@umn.edu
Thesis Advisor: Scott
McIvor
Year entered: 2005
Degrees received:
B.A., Biochemistry, Smith College, Northampton, MA 1995
Honors and Awards:
- Graduate School Fellowship 1999-2000
- MICaB travel grant, Spring semester 2008
Thesis research:
Lentiviral gene transfer for chemoprotection and improved
chemotherapy
The goal of this project is to provide systemic
chemoprotection from
methotrexate (MTX) induced acute toxicity, using lentiviral-mediated
gene
transfer of a drug resistant dihydrofolate reductase (DHFR)
into stem cells. The
four phases of this project include: (i) constructing and
evaluating lentivirus
vectors in vitro, (ii) testing chemoprotection of the hematopoietic
system
conferred by lentivirus gene transfer in a murine transplant
model, (iii) evaluating
engraftment, long-term gene expression and chemoprotection
in a pre-clinical
canine model and (iv) MTX-mediated in vivo selective expansion
of DHFR+
human embryonic stem cell-derived blood cells in NOD/SCID/gc
mice.
Publications:
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Gori J.L., K. Podetz-Pedersen, D. Swanson, A.D. Karlen,
R. Gunther, N. Somia and R.S. McIvor. 2007. Protection
of mice from methotrexate toxicity by ex vivo
transduction using lentivirus vectors expressing drug-resistant
dihydrofolate reductase. J Pharmacol Exp Ther. In
press.
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Converse, A.D., L.R. Belur, J.L. Gori, G. Liu, F. Amaya,
E. Aguilar-Cordova, P.B. Hackett and R.S. McIvor. 2004.
Counterselection and co-delivery of transposon and transposase
functions for Sleeping Beauty mediated transposition in
cultured mammalian cells. Biosci
Rep. 24(6):577-94.
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