University of Minnesota
MICaB Graduate Program
http://micab.umn.edu
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Current Students

Thiede
Joshua Thiede

E-mail:thied022@umn.edu

Thesis Advisor: Anthony Baughn

Year entered: 2013

Degrees received:
B.S., University of Wisconsin, Madison, WI, 2013

Honors and awards:

  • MICaB student service award recipient 2016
  • Abstract for Mycobacteria 2016 conference was selected for an oral presentation
  • MICaB student representative for 2016-2017


Research:
Despite advances in modern medicine, tuberculosis continues to be one of the top ten causes of death worldwide, claiming 1.8 million lives in 2015.  The increasing prevalence of drug resistant tuberculosis infections is beginning to threaten current therapies. The drug discovery pipeline has not yielded new drugs to treat tuberculosis infections in decades, leading to a rapid depletion of treatment options. To combat the rise of drug resistance, development of novel therapeutic regimens is crucial. Repurposing and enhancing existing drugs offers a unique opportunity to prevent and cure drug resistant infections. My thesis research focuses on the development of strategies to disrupt intrinsic bacterial drug resistance in an effort to improve currently available antibiotics. Intrinsic drug resistance determines the amount of drug necessary to successfully inhibit and kill the infecting organism.  Our work has demonstrated disruption of various intrinsic drug resistance mechanisms leads to improved antitubercular activities of pyrazinamide and p-aminosalicylic acid. We have also demonstrated that targeting intrinsic resistance has the potential to counter antibiotic resistance that may have already developed.

Publications:

  • Minato, Y., Thiede J.M., Kordus, S.L., McKIveen, E.J., Turman, B.J., Baughn, A.D. 2015 Mycobacterium tuberculosis folate metabolism and the mechanistic basis for para-aminosalicylic acid susceptibility and resistance. Antimicrob. Agents Chemother. 59:5097-5106.
  • J. M. Thiede, S. L. Kordus, B. J. Turman, J. A. Buonomo, C. C. Aldrich, Y. Minato, and A. D. Baughn. Targeting intracellular p-aminobenzoic acid production potentiates the anti-tubercular action of antifolates. Sci. Rep. 6, 38083 (2016).