MICaB Graduate Program
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Department of Microbiology and Immunology
University of Arizona, 1998, Ph.D.
612-624-1912 - office
612-624-7994 - lab
C. albicans genetics and pathogenesis
My primary research focus of my laboratory is to understand how the fungal pathogen C. albicans responds to changes in the extracellular environment and how these changes are related to virulence. We have identified two distinct signal transduction pathways that govern adaptation to extracellular pH in vitro and are critical for virulence in vivo. To further this research we are addressing the following questions:
1. What are mechanistic details of these pathways and how do they relate to each other to govern pH responses?
2. What are the targets of these pathways and how do these targets affect the phenotypes seen in pathway mutants?
3. How do these pathway promote virulence?
To answer these questions, we have developed both forward and reverse genetic techniques which provide powerful approaches to address questions 1 and 2. Further, we are using an ex vivo models, including C. albicans stimulated host cell damage models, to address question 3 .
Selected Recent Publications:
- Baek YU, Martin SJ, Davis DA. 2006. Evidence for novel pH-dependent regulation of C. albicans Rim101, a direct transcriptional repressor of the cell wall -glycosidase Phr2. Eukaryotic Cell. Sept; in press.
- Granger BL, Flenniken ML, Davis DA, Mitchell AP, Cutler JE. 2005. Yeast wall protein 1 of Candida albicans. Microbiology. May;151(Pt 5):1631-44.
- Kullas AL, Li M, Davis DA. 2004. Snf7p, a component of the ESCRT-III protein complex, is an upstream member of the RIM101 pathway in Candida albicans. Eukaryot Cell Dec;3(6):1609-18.
- Bensen ES, Martin SJ, Li M, Berman J, Davis DA. 2004. Transcriptional profiling in Candida albicans reveals new adaptive responses to extracellular pH and functions for Rim101p. Mol Microbiol Dec;54(5):1335-51.
- Li M, Martin SJ, Bruno VM, Mitchell AP, Davis DA. 2004. Candida albicans Rim13p, a protease required for Rim101p processing at acidic and alkaline pHs. Eukaryot Cell Jun;3(3):741-51.
- Davis D. 2003. Adaptation to environmental pH in Candida albicans and its relation to pathogenesis. Curr Genet. Oct;44(1):1-7.
- Nobile CJ, Bruno VM, Richard ML, Davis DA, Mitchell AP. 2003. Genetic control of chlamydospore formation in Candida albicans. Microbiology. Dec;149(Pt 12):3629-37.
- Spreghini E, Davis DA, Subaran R, Kim M, Mitchell AP. 2003. Roles of Candida albicans Dfg5p and Dcw1p cell surface proteins in growth and hypha formation. Eukaryot Cell. Aug;2(4):746-55.
- Davis DA, Bruno VM, Loza L, Filler SG, Mitchell AP. 2002. Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis. Genetics Dec;162(4):1573-81.
- Cruz MC, Goldstein AL, Blankenship JR, Del Poeta M, Davis D, Cardenas ME, Perfect JR, McCusker JH, Heitman J. 2002. Calcineurin is essential for survival during membrane stress in Candida albicans. EMBO J. Feb 15;21(4):546-59.
- Cognetti D, Davis D, Sturtevant J. 2002. The Candida albicans 14-3-3 gene, BMH1, is essential for growth. Yeast Jan 15;19(1):55-67.
- Whitacre J, Davis D, Toenjes K, Brower S, Adams A. 2001. Generation of an isogenic collection of yeast actin mutants and identification of three interrelated phenotypes. Genetics Feb;157(2):533-43.
- Davis D, Edwards JE Jr, Mitchell AP, Ibrahim AS. 2000. Candida albicans RIM101 pH response pathway is required for host-pathogen interactions. Infection and Immunity 68:10 5953-5959.
- Davis D, Wilson RB, Mitchell AP. 2000. RIM101-dependent and-independent pathways govern pH responses in Candida albicans. Molecular and Cellular Biology 20:3 971-978.
- Wilson RB, Davis D, Enloe BM, Mitchell AP. 2000. A recyclable Candida albicans URA3 cassette for PCR product-directed gene disruptions. . Yeast 16:1 65-70.
- Wilson RB, Davis D, Mitchell AP. 1999. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions Journal of Bacteriology 181:6 1868-1874.