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Nobuaki Kikyo, Ph.D.
Assistant Professor
Department of Medicine
Tokyo University Medical School, 1993, Ph.D.
kikyo001@umn.edu
612-624-0498 - office
612-625-1452 - lab
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Research Interests:
Nuclear remodeling, stem cells and cancer
Differentiated somatic nuclei can dedifferentiate in egg
cytoplasm and redifferentiate during early development as
proven by somatic cells nuclear cloning. Our long-term goal
is to understand the reprogramming mechanism of somatic nuclei
at the molecular level. This study will contribute to future
regeneration/transplantation medicine avoiding ethically controversial
embryonic cells. One of our two projects concerns reversible
disassembly of somatic nucleoli by the frog germ cell proteins
FRGY2a and FRGY2 (Gonda et al, 2003). Since nucleoli sequester
many proteins essential for cell proliferation, such as telomerase,
nucleostemin and the p53 regulator ARF, this research will
also contribute to our understanding of cancer progression.
The second project investigates reprogramming of histone modifications
and heterochromatin in nuclear cloning. This study of epigenetic
gene regulation is important for cancer biology as well. Thus,
investigation of nuclear reprogramming significantly benefits
progress of cancer research.
Selected Recent Publications:
- Romanova, L., Katoku-Kikyo, N., Kellner, S. and Kikyo,
N. Novel role of nucleostemin in the maintenance of nucleolar
architecture and integrity of small nucleolar ribonucleoproteins
and the telomerase complex. J Biol Chem. In press.
- Kellner, S., and Kikyo, N. Transcriptional regulation
of the Oct4 gene, a master gene for pluripotency Histology
and Histopathology. In press.
- Romanova, L., Grand, A., Zhang, L., Rayner, S., Katoku-Kikyo,
N. and Kikyo, N. 2009. Critical role of nucleostemin in
pre-rRNA processing. J
Biol Chem, 284, 4968-77.
- Kikyo, N. 2008. Approaches to identify nuclear reprogramming
factors from embryonic stem cell biology and somatic cell
nuclear cloning. Stem Cells and Regenerative Medicine, 45-62.
- Zhang, L., Rayner, S., Katoku-Kikyo, N., Romanova. L.,
and Kikyo N. 2007. Successful co-immunoprecipitation of
Oct4 and Nanog using cross-linking. Biochem
Biophys Res Comm, 361, 611-614.
- Gonda, K. and Kikyo, N. 2006. Nuclear remodeling assay
in Xenopus egg extract. Method
Mol Biol 348, 247-258
- Tamada, H., Thuan, N. V., Reed, P., Nelson, D., Katoku-Kikyo,
N., Wudel,J., Wakayama, T. and Kikyo, N. 2006. Chromatin
decondensation and nuclear reprogramming by nucleoplasmin. Mol
Cell Biol 26, 1259-71.
- Gonda, K., Wudel, J., Nelson, D., Katoku-Kikyo, N., Reed,
P, Tamada, H. and Kikyo, N. 2006. Requirement of the protein
B23 for nucleolar disassembly induced by the FRGY2a family
proteins. J
Biol Chem 281, 8153-60.
- Gonda, K., Fowler, J., Katoku-Kikyo, N., Haroldson, J.,
Wudel, J. and Kikyo, N. 2003. Reversible disassembly of
somatic nucleoli by the germ cell proteins FRGY2a and FRGY2b.
Nature
Cell Biol 5, 205-10.
- Kikyo, N., Wade, P. A., Guschin, D., Ge, H. and Wolffe,
A. P. 2000. Active remodeling of somatic nuclei in egg cytoplasm
by the nucleosomal ATPase ISWI. Science
289, 2360-2362.
Last updated: August 10, 2009 |
