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Nicola Philpott, Ph.D.
Assistant Professor
Department of Medicine
University of Leeds, UK, 1998
philpott@umn.edu
612-624-0776 - office
612-624-1011 - lab
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Research Interests:
Viral vectors for gene therapy
Viral vectors are very efficient gene transfer tools and are
currently being used in many gene therapy trials for the treatment
of hereditary disease, infectious diseases and cancer. Long
term expression of a transgene often requires integration
of the gene into the human genome however random insertion
can lead to adverse side effects. We are interested in improving
the safety of gene therapy vectors by blocking random integration
while retaining effective gene transfer. We have 2 areas of
study: 1) We are developing Adeno-associated viral vectors
(AAV) that integrate into a specific site within the human
genome that is not associated with any pathogenicity. As well
as elucidating the mechanism of AAV Rep-mediated integration,
we are using this targeted integration strategy to achieve
stable transgene expression in dividing cells for gene therapy.
2) Non-integrating lentiviral vectors (NILVs) can be used
for stable gene delivery to non-dividing cells. We are currently
investigating the use of NILVs to achieve prolonged transgene
expression in post-mitotic tissue for the treatment of genetic
disease.
Selected Recent Publications:
- Apolonia, L., Waddington, S. N., Fernandes, C., Ward,
N., J., Bouma, G., Blundell, M. P., Thrasher, A. J., Collins,
M. K., Philpott, N. J. 2007. Stable gene transfer to muscle
using non-integrating lentiviral vectors. Molecular
Therapy 15(11): p1947-54.
- Philpott, N. J. 2007. Viral vectors for gene therapy -
review. In: Encyclopaedia of Life Sciences September. John
Wiley & Sons, Ltd: Chichester http://www.els.net/ [DOI:
10.1002/9780470015902.a0020707]
- Schoggins, J. W., Nociari, M., Philpott, N., Falck-Pedersen,
E. 2005. Influence of fiber detargeting on adenovirus mediated
innate and adaptive immune activation. Journal
of Virology 79(18): p11627-37.
- Philpott, N. J., Nociari, M., Elkon, K. B., Falck-Pedersen,
E. 2004. Adenovirus-induced maturation of dendritic cells
through a PI3 kinase-mediated TNF-alpha induction pathway.
PNAS
101(16): p6200-5.
- Philpott, N. J., Gomos, J., Falck-Pedersen, E. 2004. Transgene
expression after Rep-mediated site-specific integration
into chromosome 19. Human
Gene Therapy 15(1): p47-61.
- Cannon, M., Philpott, N. J., Cesarman, E. 2003. The KSHV
G protein-coupled receptor has broad signaling effects in
primary effusion lymphoma cells. Journal
of Virology 77(1): p57-67.
- Philpott, N. J., Gomos, J., Berns, K. I., Falck-Pedersen,
E. 2002. A p5 integration efficiency element mediates Rep-dependent
integration into AAVS1 at chromosome 19. PNAS
99(19): p12381-5.
- Philpott, N. J., Giraud-Wali, C., Dupuis, C., Gomos, J.,
Hamilton, H., Berns, K. I., Falck-Pedersen, E. 2002. Efficient
integration of recombinant adeno-associated virus DNA vectors
requires a p5-rep sequence in cis. Journal
of Virology 76(11): p5411-21.
- Trevejo, J., Marino, M., Philpott, N., Josien, R., Richards,
E., Elkon, K., Falck-Pedersen, E. 2001. TNF-alpha-dependent
maturation of local dendritic cells is critical for activating
the adaptive immune response. PNAS
98(21): p12162-7.
Last modified on: June 23, 2008
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