University of Minnesota
MICaB Graduate Program
http://micab.umn.edu
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MICaB Faculty

David A. Potter
David A. Potter, M.D., Ph.D.

Associate Professor

Department of Medicine

Johns Hopkins University, 1987, M.D., Ph.D.

612-625-8933 office
612-626-7207 lab

E-mail:dapotter@umn.edu


Research Interests:

Regulation of calpain proteases and their roles in cytoskeletal remodeling; the roles of cytochrome P450 in breast cancer progression

Three enzymatic pathways of arachidonic acid metabolism, involving cyclooxygenases, lipoxygenases and epoxygenases, have been identified in mammalian cells, but only the first two have been mechanistically linked to human cancer. The HIV protease inhibitor ritonavir is a potent inhibitor of epoxygenases that arrests the growth of breast cancer xenografts, but its mechanism of action is unknown. Epoxygenases promote the production of epoxyeicosatrienoic acids (EET’s) that activate Akt kinase. Our studies seek to determine whether epoxygenases are cancer therapeutic targets. The hypothesis to be tested is that epoxygenase activation promotes breast cancer progression by promoting Akt phosphorylation and cancer cell survival. Based on our observations we are asking the following questions: What are the molecular mechanisms by which epoxygenases cause growth dysregulation in breast cancer? Do epoxygenases enhance the transforming activities of oncogenes in mammary carcinoma? Do epoxygenase pathways require Hsp90 activity for cancer cell survival? Targeted lipidomics will be used to assay EET regio- and stereoisomers. These studies will promote further development of epoxygenases as targets for breast cancer therapeutics.

Selected Recent Publications:

 

  • Srirangam, A., R. Mitra, M. Wang, J. C. Gorski, S. Badve, L. A. Baldridge, J. Hamilton, H. Kishimoto, J. Hawes, L. Li, J. S. Blum, D. B. Donner, G. W. Sledge , H. Nakshatri and D. A. Potter.  2006.  Effects of HIV Protease Inhibitor Ritonavir on Akt-Regulated Cell Proliferation in Breast Cancer. Clin. Cancer Res. 12: 1883-1896.
  • Narayanasamy, D., S. Mukhopadhyay, M. Palakal and D. A. Potter.  2004.  TransMiner: Mining Transitive Associations among Biological Objects from Text.  Journal of Biomedical Science.  11 (6):864-873.
  • D. A. Potter, Anjaiah Srirangam, K. A. Fiacco, D. Brocks, M. Maki , D. Acheson and I. M. Herman.  2003  Calpain Regulates Enterocyte Brush Border Actin Assembly and Pathogenic E. coli-Mediated Effacement.  J. Biol. Chem. 278 (32):30403–30412.
  • Croce, K., R. Flaumenhaft, M. Rivers, B. C. Furie, B. Furie, I. M. Herman and D. A. Potter.  1999.  Calpain regulates platelet secretion, aggregation and spreading.  J. Biol. Chem. 274:36321-36327.
  • Potter, D. A., J. S. Tirnauer, R. Janssen, D. E. Croall, C. N. Hughes, K. A. Fiacco, J.W. Mier, M. Maki  and I. M. Herman.  1998.  Calpain regulates the actin cytoskeleton during cell spreading. J. Cell Biol. 141:647-662.

 

 

Last modified on: February 6, 2007