Janet L. Schottel, Ph.D. Professor Department of Biochemistry, Molecular Biology and Biophysics Washington University, 1977, Ph.D. schot002@tc.umn.edu 612-624-6275 office 612-624-1737 lab

Research Interests:

Regulation of Gene Expression; Stress Responses; Microbial-based Cancer Therapies

One of my primary research interests focuses on the regulation of gene expression in cells in response to stresses such as desiccation, nutrient deprivation, culture density, osmotic stress, heat shock, and mechanical stress. Our approaches include studying the regulation of transcription, mRNA degradation and protein synthesis in cells grown under a variety of culture conditions.

A new interest in the lab is the development of microbial-based therapies for cancer. An attenuated strain of Salmonella enterica Typhimurium is currently used in these studies. This organism is a gram-negative facultative bacterium that can invade and divide with macrophages and other cell types and thrives in hypoxic areas of tumors. Previous reports have indicated that administration of this organism significantly reduced tumor size and number in mouse models of metastatic osteosarcoma, primary neuroblastoma and liver adenocarcinoma. Current work is focused on optimizing the cancer suppressing activity of this bacterium by expressing various cytokine genes and determining the most effective protocol for administration of this organism to the mice with potential future applications to humans.

Selected Recent Publications:

• Scherber, C., J.L. Schottel and A. Aksan. 2009. Membrane phase behavior of Escherichia coli during desiccation, rehydration, and growth recovery. BBA-Biomembranes, in press.
• Schottel, J.L., Orwin, .M., Anderson, C.R. and Flickinger, M.C. 2008. Spatial expression of a mercury-inducible green fluorescent protein within a nanoporus latex-based biosensor coating. J. Ind. Microbiol. Biotechnol. 35(4):283-90.
• Flickinger, M.C., Schottel, J.L., Bond, D.R., Aksan, A. and Scriven, L.E. 2007. Painting and printing lliving bacteria: engineering nanoporus biocatalytic coatings to preserve microbial viability and intensify reactivity. Biotechnol. Prog. 23(1):2-17.
• Schottel, J., C. Anderson and M. Flickinger. 2005. Mercury biosensors: Spatial expression of mercury-induced green fluorescent protein or luciferase in Escherichia coli immobilized in latex biocatalytic coatings. Cryobiology 51:384.
• Ryan, A.D., L.L. Kinkel, and J.L. Schottel. 2004. Effect of Pathogen Isolate, Potato Cultivar, and Antagonist Strain on Potato Scab Severity and Biological Control. Biocontrol Science and Technology, 14:301-311.
• Flickinger, M.C., S. Charaniya, C. Solheid, C.R. Anderson, O.K. Lyngberg, H. Ge, J.L. Schottel, and L.E. Scriven. 2004. Catalytic Coatings - Multi-Layer, Permeable, Reactive Latex Coatings Containing Living Bacteria: Applications for Biosensors, Bio-Electronic Devices, and Biocatalysis. European Coating Conference, Smart Coatings III; Berlin, Germany.
• Schottel, J.L., K. Shimizu, and L.L. Kinkel. 2001. Relationships of in vitro pathogen inhibition and soil colonization to potato scab biocontrol by antagonistic Streptomyces spp. Biol. Control. 20:102-112.
• Neeno-Eckwall, E.C., L.L. Kinkel, and J.L. Schottel. 2001. Competition and antibiosis in the biological control of potato scab. Can. J. Microbiol. 47:332-340.
• Lyngberg, O.K., D.J. Stemke, J.L. Schottel, and M.C. Flickinger. 1999. A single-use luciferase-based mercury biosensor using Escherichia coli HB101 immobilized in a latex copolymer film. J. Ind. Microbiol. Biotechnol. 23:668-676.
• Thiagarajan, V.S., Z. Huang, L.E. Scriven, J.L. Schottel, and M.C. Flickinger. 1999. Microstructure of a biocatalytic latex coating containing viable Escherichia coli cells. J. Coll. Inter. Sci. 215:244-257.
• Lyngberg, O.K., V. Thiagarajan, D.J. Stemke, J.L. Schottel, L.E. Scriven and M.C. Flickinger. 1999. A patch coating method for preparing biocatalytic films of Escherichia coli. Biotechnol. Bioeng. 62:44-55.
• Neeno-Eckwall, E.C. and J.L. Schottel. 1999. Occurrence of antibiotic resistance in the biological control of potato scab disease. Biol. Control 16:199-208.
• Kinkel, L.L., Bowers, J.H., Shimizu, K., Neeno-Eckwall, E.C. and Schottel, J.L. 1998. Quantitative Relationships Among Thaxtomin A Production, Potato Scab Severity, and Fatty Acid Composition in Streptomyces. Can. J. Microbiol. 44:768-776.
• Kuzj, A.E.S., P.S. Medberry, and J.L. Schottel. 1998. Stationary phase, amino acid limitation and recovery from stationary phase modulate the stability and translation of chloramphenicol acetyltransferase mRNA and total mRNA in Escherichia coli. Microbiol. 144:739-750.
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Last modified on: August 18, 2009