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Bruce K.Walcheck, Ph.D.
Associate Professor
Department of Veterinary Pathobiology
Montana State University, 1994, Ph.D.
walch003@umn.edu
612-624-2282 office
612-624-9741 lab
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Research Interests:
Cell adhesion: leukocyte migration
Our research focus pertains to the mechanisms of leukocyte
migration from the blood into lymphoid organs and sites of
inflammation. Leukocytes disciminate between tissue sites,
in part, by adhesion molecules expressed on the leukocyte
cell surface and on the endothelial cell lining of the vascular
wall. Our work primarily focuses on the selectin and integrin
families of adhesion proteins and their ligands. These adhesion
molecules perform more than just a passive role of adhesion,
they also regulate their affinity, expression levels, and
numerous intracellular events. For instance, L-selectin receptors
are rapidly cleaved (within seconds to minutes) from the cell
surface of leukocytes following cell activation and ligand
interaction. We are investigating the intermolecular interactions
within the cell that participate in this process. In our research,
we use state-of-art equipment an techniques, including flow
cytometry and sorting, videomicroscopy and comptuer-based
image analysis, molecular biology, protein biochemistry, and
monoclonal antibody production.
Walcheck
lab
Selected Recent Publications:
- Schaff U, Mattila PE, Simon SI, Walcheck B. 2007. Neutrophil
adhesion to E- selectin under shear promotes the redistribution
and co-clustering of ADAM17 and its proteolytic substrate
L-selectin. J
Leukoc Biol. In press.
- Bell J., Herrera AH. Li, Y., and Walcheck B. 2007. Role
of ADAM17 in the ectodomain shedding of TNF-alpha and its
receptors by neutrophils and macrophages. J.
Leuko. Biol. 82:173-176.
- Ni, Z. and Walcheck, B. 2007. Varied levels of reactivity
by different E-selectin/Fc constructs with cutaneous lymphocyte-associated
antigen (CLA)+ CD4+ T cells. Immunol.
Lett. 108:179.
- Li, Y., Brazzell, JL., Herrera, AH., Walcheck, B. 2006.
ADAM17 deficiency by
mature neutrophils has differential effects on L-selectin
shedding. Blood
108(7):2275-9.
- Ni Z, Campbell JJ, Niehans G, Walcheck B. 2006. The Monoclonal
Antibody CHO-131 Identifies a Subset of CutaneousLymphocyte-Associated
Antigen T Cells Enriched in P-Selectin-Binding Cells. J
Immunol. 177(7):4742-8.
- Walcheck, B., Herrera, A.H., St. Hill, C.A., Mattila,
P., Whitney, A.R., and DeLeo, F.R. 2006. ADAM17 activity
during human neutrophil activation and apoptosis. Eur.
J. Immunol. 36:968-76.
- Mattila, P., Green, C.E., Schaff, U., Simon, S.I., and
Walcheck, B. 2005. L-selectin clustering involves altered
lipid phase partitioning and is regulated by cytoskeletal
anchorage. Amer.
J. Physiol. Cell Physiol. 289:323.
- St. Hill, C.A., Bullard, K.M. and Walcheck, B. 2005. Expression
of the high-affinity selectin glycan ligand C2-O-sLeX by
colon carcinoma cells. Cancer
Lett. 217:105.
- Walcheck, B., Alexander, S.R., St. Hill, C.A., and Matala,
E. 2003. ADAM-17-independent shedding of L-selectin. J.
Leukoc. Biol. 74:389.
- Catherine St. Hill, Shelia R. Alexander, and Bruce Walcheck.
2003. Indirect capture augments leukocyte accumulation on
P-selectin in flowing whole blood. J.
Leukoc. Biol. 73: 464.
- Walcheck, B., Leppanen, A., Cummings, RD., Knibbs, RN.,
Stoolman, LM., Alexander, SR., Mattila, PE., and McEver,
RP. 2002. The monoclonal antibody CHO-131 binds to a core
2 O-glycan terminated with sialyl-Lewis x, which is a functional
glycan ligand for P-selectin. Blood
99:4064.
- Matala, E., Alexander, S.R., Kishimoto T.K., and Walcheck
B. 2001. The cytoplasmic domain of L-selectin participates
in regulating L-selectin endoproteolysis.
J. Immunol. 167:1617.
- Alexander, S.R., Kishimoto, T.K., and Walcheck, B. 2000.
Effects of selective protein kinase C inhibitors on the
proteolytic downregulation of L-selectin from chemoattractant-activated
neutrophils. J.
Leuk. Biol. 67:415.
- Kahn, J., B. Walcheck, G. Migaki, M.A. Jutila, and T.K.
Kishimoto. (1998) Calmodulin regulates L-selection adhesion
molecule expression and function through a protease-dependent
mechansim. Cell
92:809.
- Walcheck, B., J. Kahn, J.M. Fisher, B.B. Wang, R.S. Fisk,
D.G. Payan, C. Feehan, R. Betageri, K. Darlak, A.F. Spatola,
and T.K. Kishimoto. (1996) Neutophil rolling altered by
inhibition of L-selectin shedding in vitro.
Nature 380:720.
- Walcheck, B., K.L. Moore, R.P. McEver, and T.K. Kishimoto.
(1996) Neutrophil-neutrophil interactions under hydrodynamic
shear stress involve L-selectin and PSGL-1: A mechanism
that amplifies initial leukocyte accumulation on P-selectin
in vitro. J.
Clin. Invest. 98:1081.
Last modified on: November 15, 2007 |